Journal article
Ahad Hossain, Joydeb Majumder, Cheng Bi, Fang Huang, G. Chopra
2021
2021
Semantic Scholar
DOI
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APA
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Hossain, A., Majumder, J., Bi, C., Huang, F., & Chopra, G. (2021). Stable and Reversible Functionalization and Super-Resolution Microscopy of Live Cell Membranes.
Chicago/Turabian
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Hossain, Ahad, Joydeb Majumder, Cheng Bi, Fang Huang, and G. Chopra. “Stable and Reversible Functionalization and Super-Resolution Microscopy of Live Cell Membranes” (2021).
MLA
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Hossain, Ahad, et al. Stable and Reversible Functionalization and Super-Resolution Microscopy of Live Cell Membranes. 2021.
BibTeX Click to copy
@article{ahad2021a,
title = {Stable and Reversible Functionalization and Super-Resolution Microscopy of Live Cell Membranes},
year = {2021},
author = {Hossain, Ahad and Majumder, Joydeb and Bi, Cheng and Huang, Fang and Chopra, G.}
}
Abstract
Live cell surface functionalization has been increasingly explored in recent years because of its emerging bio- medical and therapeutic applications. Known methods to functionalize live-cell surface includes time-consuming metabolic docking of non-natural functional groups on cell surfaces that are hard to use. Here, we report an easy and direct functionalization method for cell surface and cell-cell conjugation utilizing native phosphate groups on cell membranes. We used three-dimensional single-molecule localization microscopy to confirm that the chemical conjugate binds preferentially on the cell surface. We show an application of this method for live-cell imaging and enhancement of cell-cell interaction leading to increased T cell proliferation. Our simple and reversible cell-conjugation strategy would be widely useful to explore and optimize cell-cell contact-based proliferation for any cell-based therapeutic applications.
